earticle

영어

Hydrogenase is one of key enzymes in biological hydrogen production using various microorganisms. After it was found in Escherichia coli by Stephenson and stickland in 19311 at the first time, tremendous efforts have been done to elucidate its biological role and biochemical property. However, many things remain uncertain because its formation and maturation process follows very complicated steps and gene transcription and expression is controlled by complex regulation. Up to now, it has been known that hydrogenase 1 and 2 have only hydrogen uptake activity different to hydrogenase 32. In this study full operon of hya, encoding hydrogenase 1 of E. coli, was cloned and effect of overexpression of hydrogenase 1 in E. coli on biological hydrogen production was checked. Recombinant E. coli BL21 expressing hydrogenase 1 produced highly enhanced molecular hydrogen compared to wild type strain which produces little amount of hydrogen.