원문정보
초록
영어
Bacteria form biofilms by adhering to biotic or abiotic surfaces, causing several problems such as reducing the transport of mass and heat, increasing resistance to antibiotics, and shortening the lifetime of modules in bioindustrial fermentors. To overcome these problems, a biofilm-deficient Escherichia coli BD123 was constructed by deleting genes involved in curli biosynthesis and assembly 8(csgG-csgC), colanic acid biosynthesis and assembly 8(wcaL-wza), and type I pili biosynthesis 8(fimB-fimH). BD123 almost remained as planktonic cells under laboratory
conditions. BD123 became more sensitive to antibiotics than the wild-type E. coli MG1655: the
growth of BD123 was inhibited even with one fourth of the antibiotics needed for the growth
inhibition of MG1655. In addition, the transformation efficiency of BD123 was about 20 times
higher than that of MG1655 and the production of recombinant proteins and secretion of protein
expressed was about 16% and 25% greater in BD123, respectively. These results indicate that the
biofilm-deficient strain has several significant advantages as a host strain for biotechnology.