원문정보
초록
영어
Stability of mRNA is an important factor that directly affects the efficiency of cell-free protein synthesis. Although the half-life of mRNA in a cell-extract does not generally exceed several miniutes, in this study, we demonstrate that an appropriately designed mRNA molecule can serve as functional template for greatly extended reaction periods in a continuous-exchange cell-free protein synthesis (CECF) system. When a reaction mixture for CECF reaction was
programmed with in vitro-transcribed mRNA, continuous accumulation of protein product was observed at least for 20 hours, indicating that the mRNA molecules were effectively protected from nucleolytic enzymes present in the cell-extract. We believe that this astonishing result was due to the blockage of mRNA with continuously recycling ribosomes. We also expect that the presented results will significantly contribute to the development of efficent tools for deciphering
genetic information.