earticle

영어

Streptomyces are the well known gram positive bacteria which contribute a large number of secondary metabolites including a number of antitumor drugs. Streptomyces peucetius ATCC 27952 is reported to be only an organism till date to produce anticancer chemotherapeutic doxorubicin. Genome analysis of this strain shows the presence of a large number of gene clusters responsible for doxorubicin production. We describe here the construction of disruption vector by using pKC1139 to delete doxorubicin biosynthetic gene clusters from S. peucetius ATCC 27952.