earticle

영어

The sequence analysis of the metabolically rich genome of Streptomyces peucetius ATCC 27952 revealed a 2199 bp sesquiterpene cyclase encoding gene from germacradienol synthase gene cluster named as Sp-gS whose putative function is Germacradienol/Geosmin synthase. The amino acid sequence of Sp-gS shows 66 % identity with SAV2163 (geoA) gene from Streptomyces avernitilis MA-4680 and which produces 4,8a-dimethyl-decahydronaphthalen-4a-ol (geosmin). The gene encoding sesquiterpene cyclase from Streptomyces peucetius ATCC 27952 has been amplified, cloned and expressed in E. coli BL21 (DE3).