earticle

영어

CoenzymeQ10 is a promising nutraceutical supplement that is currently produced using chemical and biotechnological methods from plant materials. While wildtype E. coli produces CoQ8 as an electron carrier in the electron transport pathway, direct production of CoQ10 from glucose is possible in recombinant E. coli expressing dps gene coding for decaprenyl diphosphate synthase. However, the amount of accumulated CoQ10 in the recombinant E .coli strain is not high enough for commercial production. Prior studies reported that overexpression of
endogenous genes (dxs and idi) enhanced the production of lycopene in E. coli by increasing metabolic fluxes in the isoprenoid synthesis pathway. We hypothesized that overexpression of dxs and idi could increase CoQ8 synthesis in E. coli because both lycopene and CoQ8 synthesis require precursors from the isoprenoid synthesis pathway. As such, we investigated the effects of dxs and idi overexpression on CoQ8 production in E. coli. Specifically, we constructed four expression cassettes containing dxs, idi, dxs+idi, idi+dxs under the control
of a strong promoter. As expected, CoQ8 production in E. coli significantly increased after introduction of the expression cassettes.