earticle

영어

Antimicrobial peptides (AMPs) are agents of the most ancient form of defense systems. With the increasing concerns about the antibiotics- resistant bacteria, the AMPs have attracted attention as alternative antibacterial agents. To date, however, few effective high-throughput approaches have been developed for designing and screening peptides. In this work, we attempted to express biologically active antimicrobial peptides in a cell-free protein synthesis system.
Through numerous approaches to enhance the efficiency of translation as well as to increase the stability of the expressed peptides, preparative amounts of active antimicrobial peptides were successfully expressed in the developed system.