원문정보
초록
영어
For the production of 1,2-propanediol, in Saccharomyces cerevisiae, pESC-URA including
two multiple cloning sites was used as yeast expression vector. The vector is under the
control of GAL promoters, GAL1 and GAL10 and the utility of the GAL promoters depends on the level of a positive activator [1],[2]. The metabolic pathway of 1,2-propanediol in yeast has been studied [3]. We selected two target gene, mgs and gldA, have important roles and cloned the target genes on pESC-URA vector. After transformation into S. cerevisiae by Li-AC method, the transformants on SDC URA- plate was selected. The concentrations of galactose and glucose were determined by expressing β-galactosidase gene on site 1 or 2 and both site 1 and 2 and assaying β -galactosidase activity [4]. As a results, S. cerevisiae containing plasmid pESC-URA
grew well under the condition 2.5:17.5 (glucose: galactose).