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Mannheimia succiniciproducens MBEL55E, a capnophilic rumen bacterium, produces succinic acid as a major fermentation product during anaerobic cultivation. M. succiniciproducens has been known to use anaplerotic pathway to produce succinic acid. In the starting point of the anaplerotic pathway, two Enzymes, phosphoenolpyruvate carboxylase(Ppc) and phosphoenolpyruvate kinase(PckA), convert phosphoenolpyruvate to oxaloacetate. We analysed
activation of both enzymes to elucidate the roles in the production of succinic acid. This study shows that PckA is 50 times stronger than Ppc in M. succiniciproducens, suggesting that PckA is a very important enzyme for producing succinic acid. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government
(MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].