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초록
영어
Pseudomonas putida KT2440 zwf, encoding G6PDH catalyzes the oxidation of glucose-6-phosphate to 6 phosphoglucolactone. Cultured in minimal medium containing glucose, northern blot analysis has shown that the zwf-1 was highly induced. However the expression level was not detected in the presence of pyruvate or succinate. We constructed a GFP-based reporter fusion using the zwf-1 gene to study the activity of the promoter. Pzwf-1 is increased in the presence of glucose. In contrast with glucose, organic acids suppress the promoter activity. A knock out mutant of HexR, a putative transcription regulator adjacent to zwf-1 gene, has constant Pzwf-1 activity in any condition. Electrophoretic mobility shift assay shows that the HexR protein binds the zwf-1 promoter region. Interestingly, the zwf-1 is also highly expressed in the presence of ROS producing materials. The Zwf induction mechanism caused by oxidative stress is not known in Pseudomonas sp. Therefore induction of zwf by oxidative stress of P. putida will be discussed. This work was supported by a NCRC grant (R15-2003-012-02002-0) and a grant (R0503443) from the BioGreen21 program