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영어

In the present study, we performed a differential proteomic analysisusing two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry to clarify the molecular mechanism for suppressive effect of chitosan oligosaccharide (CO) during differentiation of adipocyte
3T3-L1.1),2) The cell differentiation was significantly inhibited by CO at the concentration of 4 mg/mL. The protein mapping of adipocyte homogenates by 2-DE revealed that numerous protein spots were differentially altered in response to CO treatment. Out of 50 identified proteins showing significant alterations, 6 were up-regulated and 44 were down-regulated by CO treatment in comparison to control mature adipocytes. Among them, most of the proteins are associated with lipid metabolism, cytoskeleton, and redox regulation. Taken together with immunoblot analysis, it was concluded that the inhibitory effect of CO on adipocyte differentiation was mediated by C/EBPα and PPARγ pathway through significant down-regulations of important adipogenic molecules such as fatty acid binding protein and glucose transporter 4.