원문정보
Extraction of Scopoletin from Artemisia capillaris and its Antioxidative Activity
초록
영어
Artemisia capollaris is an oriental medicinal plant in Korea and it has traditionally been used as a herbal medicine for the treatment of jaundice and chronic liver disease. And its component is well known as an antioxidant and antimicrobial material. However the optimum extraction method of Artemisia capollaris has not been studied well. Scopoletin (6-methoxy-7-hydroxycoumarin) is a phenolic coumarin and a member of the phytoalexins. Also scopoletin is used as a peroxide or calcium signal during the oxidative burst. Our study was conducted to determine the contents of scopoletin and antioxidative activity of the Artemisia capollaris extracts (by electron donating
ability(EDA) from DPPH method), total polyphenol contents and flavonoid contents.Samples are extracted in water and volume of the solvent used 10 times of sample on a weight/volume basis(5g/50ml). In this experiment, we extracted scopoletin from Artemisia capollaris with
water for 1hr, 3hr and 6hr at 60℃. Quantitative analysis of extracted samples have determined by HPLC. The highest content of scopoletin was about 120mg/g in 6hr extraction. The optimum EDA(50.26%) and total polyphenol content(1.58mg/ml) were observed in 1hr extraction and its extraction was presented similar EDA(25.06%) compared with that of α-tocopherol. And the flavonoid contents were showed almost same as time change. Artemisia capollaris has sufficient values to use as a food stuff for functional foods. So we hope that this result will be used as the basic resource in developing of the beverage with Artemisia capollaris in the near future.
