원문정보
초록
영어
Three different polysaccharide-peptide complexes (PPC, named as Fr-I, Fr-II, and Fr-III) were produced by submerged mycelial culture of an entomopathogenic fungus Cordyceps sphecocephala, and their anticancer activities were investigated in human hepatocarcinoma (HepG2)1) and neuroblastoma (SK-N-SH)2) cells.The highest inhibitory effects of PPC on both
HepG2 and SK-N-SH cells were achieved with Fr-I, whereas Fr-III with low molecular weight showed lower inhibition effects. Interestingly, the inhibitory effects of the three fractions were increased after protease digestion, suggesting that the inhibitory effects resulted mainly from the carbohydrate moiety in PPC. The results of DNA fragmentation in PPC-induced apoptotic cells were confirmed by DNA ladder assay and comet assay. Our investigation also showed
that PPC-induced apoptosis of both cancer cells were associated with intracellularevents including DNA fragmentation, activation of caspase-3, modulation of Bcl-2 and Bax. We conclude that PPC has potential as a novel therapeutic agent for the treatment of both HepG2 and SK-N-SH cancer cells.