원문정보
초록
영어
A novel cathepsin B inhibitor-producing microorganism was identified based on its 16S rRNA sequence as Pseudomonas sp. PB01 (accession no. EU126129). The enzyme inhibitor productivity was investigated under various culture conditions. Both glucose and maltose proved to be the effective carbon sources for cathepsin B inhibitor production, while the mixture of organic nitrogen source was required for the optimal production. Other optimal culture conditions included temperature range between 25 and 28 oC, initial medium pH 6.6, and shaking speed 200 rpm. Under these optimal conditions, the maximum inhibitory activity from culture broth was approximately 50% after 30 h of cultivation. Kinetics study revealed that inhibitor product paralleled with cell growth. It is concluded that Pseudomonas sp. strain PB01 is a promising candidate for cathepsin B inhibitor production microorganism.