원문정보
초록
영어
In an attempt to search for novel biomarkers for monitoring diabetes prognosis, we examined the influence of the hypoglycemic fungal extracellular polysaccharides (EPS) on the differential change in pancreatic proteome and transcriptome in streptozotocin (STZ)-induced diabetic rats using 2DE-based protein mapping and oligonucleotide microarray analysis.1),2) A total of 22 overexpressed and 12 underexpressed proteins were significant between the healthy and diabetic rats, of which 26 spots were identified as the proteins whose differential expression has
previously been associated with diabetes. The microarray analysis revealed that 115 out of 1272 genes were down- or upregulated in the diabetic rats and the altered transcript levels of many of these genes were reversed after EPS treatment. In particular, ROS generation in rat islets was significantly increased after STZ treatment, thereafter EPS treatment was likely to play a preventive role in β-cell destruction mediated by STZ. Taken together, EPS may act as a potent regulator of gene expression for a wide variety of genes in diabetic rats, particularly in
anti-oxidative stress, insulin biosynthesis, and cell proliferation.