원문정보
초록
영어
To produce PHA from inexpensive substrates in bacteria, vegetable oil degrading-bacteria were isolated from rice field using enrichment cultivation. When grown on the phosphate and nitrogen limited medium containing vegetable oil as sole carbon source, Pseudomonas sp. strain DR2 was chosen because it accumulated MCL-PHA in abundance by utilizing vegetable oil. Intracellular PHA was accumulated up to 37.34% (w/w) from vegetable oil and consisted of three major 3-hydroxyalkanoate; octanoic (C8:0, 37.75% of the total 3-hydroxyalkanoate content of PHA), decanoic (C10:0, 36.74%), dodecanoic (C12:0, 11.36%). Pseudomonas sp. strain DR2 accumulated more amount of PHA than well-studied strains (C. violaceum and R. eutropha H16) when grown on vegetable oil. Interestingly, PHA production in Pseudomonas sp. strain DR2 cannot occur in either acetate- and butyrate-amended media. Pseudomonas sp. strain DR2 accumulated PHAMCL up to 23.52% (w/w) of from waste fried oil. Pseudomonas sp. strain DR2 may be very useful to produce biodegradable plastics using waste vegetable oil. This work was supported by a NCRC grant (R15-2003-012-02002-0) and a grant (R0503443) from the BioGreen21 program.