원문정보
초록
영어
The me chanisms responsible for alcohol-induced liver disease are clearly unknown. These effects of ethanol are prominent in the liver which is the major site of ethanol metabolism in the body. Liver injury is the most common response to alcohol intake, and chronic alcohol consumption generates alcoholic liver disease such as hepatic steatosis, hepatitis and cirrhosis. Alcoholic liver disease re sults fr om me tabolism co nfusion and hep atocyte d eath by ROS. Ethanol metabolism alters the cytosolic and mitochondrial redox states and disrupts
the functions of various metabolic pathways, and promotes oxidative stress both
by increasing ROS formation and by decreasing cellular defense mechanisms. This study evaluated hepatocyte protective effect and reactive oxygen species (ROS) scavenging ability of natural herbs on excessive exposure to alcohol. Natural herbs were extracted by various solvent, and then extraction yield was measured. The cells were cultured at 37℃ in 5% CO₂incubator. After ethanol treatment, hepatocyte was incubated, at once natural herbs were administrated. He patocy te p rotective ef fe ct was e valuated b y ce ll viabil ity thro ugh MTT
assay and ROS scavenging ability was determined by FACs after fluorescent dyeing.