원문정보
초록
영어
Streptococcus mutans, the major oral pathogen, produces glucan that has α-(1→3), α-(1→6),
and α-(1→4)-glycosidic linkages and this glucan forms dental plaque. In this study, a hybrid
enzyme having both dextranase and maltohexaose-producing amylase activities was constructed to apply for the removal of dental plaque or retarding formation of it. Dextranase (Dex2) and maltohexaose-producing amylase (G6-Amy) gene were cloned from Arthrobacter oxydanas and Klebsiella pneumoniae, for respectively and then ligated with each other. The open reading frame of the constructed dex2-G6Amy hybrid gene consists of 3,759 bp encoding 1,253 amino acid residues. The gene was ligated with pRSET expression vector and transformed to Escherichia coli BL21 (DE3) pLysS for enzyme expression. Expressed enzymes were purified
by application to Ni Sepharose High Performance column. The purified dex2-G6Amy enzyme
has the dextranolytic and amylolytic bifunctional activities.