원문정보
초록
영어
Daidzein is diphenolic phytoestrogen compound found in numerous plants and soybean .Hydroxylated products have been reported to have potent antioxid ant properties that contributes to their cholesterol lowering effects,cardiovascular protection ,antitumor effects and anticarcinogenic properties .Compounds with the ortho-dihydroxy groups are known to exhibit anti-inflamatory and anti-allergic activity and to express anticarcinogenic properties due to the inhibition of protein tyrosin kinase to a potent tyrosinase inhibitor of lipoxygenases .Hydroxylation of the aromatic compounds by using chemical synthesis method is difficult and involves diverse reaction steps.Since the conversion of a non activated carbon -hydrogen bond by the microbial enzyme is of increasing interest .Hydroxylation of daidzein by recombinant streptomyces avermitilis was examined.Strucuture analyisis of the reactive products was determined using gas chromatography mass spectrometry(GC-MASS).Recombinant streptomyces avermitilis is highy regioselective to give 7,3',4'-trihydroxyisoflavone.Quantification analysis of the
3'-hydroxylated daidzein were performed by HPLC.