원문정보
초록
영어
The formation of β-carotene detergent micelles and their conversion into retinal by recombinant human β,β-carotene 15,15’-monooxygenase was optimized under aqueous conditions. Toluene was the most hydrophobic among the organic solvents tested; thus, it was used to dissolve β-carotene, which is a hydrophobic compound. Tween 80 was selected as the detergent because it supported the highest level of retinal production among all of the detergents tested. The maximum production of retinal was achieved in detergent micelles containing 200 mg/L β-carotene and 2.4% (w/v) Tween 80. Under these conditions, the recombinant enzyme produced 98 mg/L retinal within 20 h with a conversion yield of 49% (w/w). The amount of retinal produced, which is the highest ever reported, is a result of the ability of our system to dissolve large amounts of β- carotene.