원문정보
초록
영어
An anaerobic glucose metabolism of hydrogen–producing Clostridium tyrobutyricum was investigated at variable initial glucose concentrations (27.8 – 333.6 mM) under batch cultivation. As an understanding of metabolic regulations was required to provide guidance or information for further gene manipulation, this was tried in order to determine carbon and electron fluxes by C. tyrobutyricum under a glucose catabolism. Then carbon material and reduction balances were adequately applied to estimate the carbon–flux distribution. Metabolic flux analysis (MFA) was also successfully employed to provide qualitative information and insight for an effective metabolic design. At the initial glucose concentration of 222.4 mM, the maximum yield of H2 was attained as 2.05 mol H2/mol glucose with the concomitant production of butyrate and a high H2/CO2 ratio.