earticle

영어

Diabetes mellitus has reached epidemic proportions and affects more than 170 million individuals worldwide [1]. Cardiovascular events and microvascular complications are major cause for morbidity and mortality of the patients with diabetes [2]. In this study, we compared the protein expression of human serum in healthy controls (n = 30) and type 2 diabetic patients without complications (n = 30) using two-dimensional electrophoresis (2-DE). Differentially-expressed proteins were identified with ESI-Q-TOF MS/MS. In patients, three proteins were up-regulated more than 2-fold (serum amyloid P component, apolipoprotein CIII, and ficolin-3) and three proteins (adiponectin precursor, complement factor I light chain, and histidine-rich glycoprotein) were down-regulated less than 50% compared to those in control. Ficolin-3 was higher in the serum of type 2 diabetic patients than in healthy subjects, and decreased during the development of diabetic nephropathy. These results imply that differentially-expressed proteins in type 2 diabetic patients may provide further information on the risk of developing diabetic complications.