earticle

영어

Hyperglycemia is recognized to be the key factor driving renal functional and pathological changes in diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggest the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN is not fully understood. In this study, to overcome the more
completely DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HK-2 cell cellular proteins were identified by ESI-Q-TOF MS/MS. The changed protein expression will provide greater understanding of DN pathways in HK-2 cell at the high concentration of glucose.