원문정보
초록
영어
The surface carbohydrate antigens on pig endothelial cells are causing immune responses in transplantation of pig organs into humans. Therefore, analysis of the carbohydrates expressed on the pig organs is of importance to predict and overcome the potential immune rejection in xenotransplantation. In this study, total N-glycans from membrane glycoproteins derived from specific pathogen-free miniature pig kidney are relatively quantified by matrix-assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and normal-phase high performance liquid chromatography (NP-HPLC) profiling after exoglycosidase digestion. The quantification results obtained fromMALDI-TOF MS and NP-HPLC indicated that the major alpha-Gal epitopes were over 30% of the total neutral N-glycans. Electrospray ionization (ESI) MS/MS was employed for the structural analysis of N-glycans from pig kidney
cell membrane glycoproteins. We were able to identify various non-Gal carbohydrate antigens, including Hangenutziu-Deicher (H-D) antigens (NeuGc-Gal-GlcNAc) as well as Gal1-3LewisX (Gal-Gal-(Fuc)GlcNAc) epitopes. Over 100 N-glycans, including sialylated and neutral types, were identified. This quantitative and qualitative structural information on carbohydrate antigens from pig kidney will be critical in overcoming the immunological barriers in clinical
alpha1,3-galactosyltransferase gene-knockout pig-to-primate xenotransplantation.