원문정보
초록
영어
Cisplatin (cis-diammindichloroplatinum Ⅱ), a commonly used chemotherapeutic agent for the treatment of solid tumors, has a major limitation as a cancer drug because of its nephrotoxicity. Decursin isolated from the root of Angelica gigas is known to be a PKC activator. Our previous studies showed decursin mediated protection of cisplatin-induced nephrotoxicity in rats. Nephrotoxicity by cisplatin involves necrosis as well as apoptosis of renal tubular cells. In this study, we examined whether decursin ameliorates cisplatin-induced nephrotoxicity in cell viability and oxidative stress in renal mesangial cells (RMCs) and primary mouse kidney cell cultures, including proximal tubular cells. We also compared the effects of decursin with N-acetylcysteine (NAC) which is known as a strong antioxidant. Cisplatin-treated cells showed significant increase in cellular ROS levels and cell death. In contrast, pretreatment of cells with decursin recovered the rise of ROS levels and influenced cisplatin-induced apoptosis. Taken together, our data indicate that decursin has a protective effect against cisplatin-induced nephrotoxicity in renal mesangial cells and proximal tubular cells.