원문정보
초록
영어
In order to develop a nanoparticle which has specificity at tumor site, a biocompatible polysaccharide was employed. The complex formed nanoparticle easily. The cationic polymer has cytotoxicity,1) but the nanoparticle from the conjugate with vitamin slightly reduced the toxicity. The polysaccharide which can be degraded by an enzyme from tumor site (BP) covered with cationic polymer.2) The cytotoxicity of the nanoparticle measured by MTT assay
depended on the enzyme concentration. The interaction between the nanoparticle and cancer cells was observed by confocal microscopy as a function of BP concentration. Although the cell cytosole was perfectly stained by the red color (RI-TC labeling nanoparticles) in the presence of the enzyme, RI-TC labeling nanoparticles only stained in the cell membrane without the enzyme. The result indicated that BP can enhance the specificity of tumor site due to inhibit the
internalization of the nanoparticle in the normal tissue.