원문정보
초록
영어
Adipogenic differentiation needs many factors to regulate protein or RNA expression. Among them, factor to regulate extracelluar matrix were confirmed, which is expected to affect adipogenic differentiation. The protein profiles from the adipocytes differentiated from hMSC were determined by 2-D gel electrophoresis (2-DE), and the differentially expressed proteins were
identified by MALDI-TOF and ESI-Q-TOF MS/MS analysis. Among these, the three up-regulated proteins (Calgizzarin, Kallikrein-9, and Ubiquitin) were newly identified from differentiated hMSC, which might prove to be useful biomarkers for the differentiation of hMSC into adipocytes. Over expression of Kallikrein-9 was reconfirmed in adipogenic cells by RT-PCR, Western blotting.
This protein was expected to play important roles for adipogenic-differentiation which will be able to help understanding of differentiation related in its extracellular matrix (ECM) not in signal transduction