원문정보
초록
영어
The main goal of this project is to study in vitro construction and in vivo application of combined skin substitutes based on the uses of cell populated gelatin and fibrin. We prepared skin equivalents with cell populated gelatin and fibrin, and then performed immunohistochemistry (IHC) analysis with bio-markers to evaluate the artificial skin in vitro and in vivo. Skin equivalent
models were prepared by combining gelatin-based dermal substitutes and fibrin-based cultured epithelium and by raising them up to the air-liquid interface. Skin substitute models were evaluated by marker proteins specific to individual dermis and epidermis. The basal membrane protein, type IV collagen and cytokeratin 14 were expressed in parallel with the basal layer protein on both skin substitute models. Also two spinous layer proteins, involucrin and
cytokerain 10 were stained. These results suggest that the skin equivalents combined with cell populated gelatin and fibrin differentiate well in vitro and in vivo and may be applicable as new skin substitutes.