원문정보
초록
영어
Defects of articular cartilage can be resolved using mesenchymal stem cells (MSCs) isolated from umbilical cord blood (UCB), which have differentiation capability into chondrocytes.1) In this study, chondrogenesis of MSCs in bioabsorbable membrane was investigated to apply in repairing damaged articular cartilage. MSCs isolated from UCB and characterized by
fluorescence-activated cell sorter (FACS) were cultured in bioabsorbable membrane so that they could be expanded in three dimensional environment. Chondrogenesis of MSCs was performed in chondrogenic medium with transforming growth factor-β3 (TGF-β3). The location of MSCs in membrane could be observed by scanning electron microscope (SEM). Moreover, the
MSCs in the membrane could also be visualized by their intense green fluorescence using confocal microscopy since enhanced green fluorescent protein (EGFP) was transformed into the MSCs. Chondrogenesis of MSCs was verified by Alcian blue, Safranin O staining and RT-PCR. These results show that MSCs can successfully differentiate into chondrocytes in
bioabsorbable membrane. In addition, chondrogenic potential of MSCs can be utilized for the repair of injured articular cartilage by cell-based therapy.