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Angelica Gigas extracts were prepared by water boiling, supercritical fluid, and ethanol extraction. Supercritical fluid extract was performed at pressure of 200-400 bar and at temperature of 40-70℃. Inhibitory effect and mechanism of skin aging by Angelica Gigas extracts were investigated using proteome analysis. The human fibroblast were cultured in Dulbecco's Modified Eagle's Medium(DMEM) with or without extracts. The proteome of cultured human
fibroblast were profiled using 2D-PAGE. Isoelectric focusing was carried out using commercial immobiline dry strips 3-10L 13cm. SDS-PAGE was performed using 18x24cm, 11% acrylamide gel1). Gels were stained with silver nitrate, analyzed by image master 2D software program. In conclusion, we could identify that many factors such as collagen, integrin beta-1, which affect the skin aging, were regulated by Angelica Gigas extracts.