원문정보
초록
영어
Pipefish, Syngnathus schlegeli (PF) a marine teleost fish, is well known not only for its special medicinal composition and used as one of the most famous and expensive materials of traditional Chinese medicine. It was extracted with water (PFW), methanol (PFM) and ethanol (PFE), respectively and evaluated by various antioxidant assays. The including total antioxidant, DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging, and alkyl radical scavenging. Further, the ROS level was detected using a fluorescence probe,
2’,7’-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on human fibrosarcoma cell (RAW264.7). Those various antioxidant activities were compared tostandard eantioxidants such as α -tocopherol. Among PFM exhibited the highest antioxidant activity in linoleic acid system, effective reducing power, DPPH radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, alkyl radical scavenging, inhibitory intracellular ROS. Furthermore, MTT assay showed no cytotoxicity on human embryonic lung fibroblasts cell line (MRC-5) and
Murine Leukemia Macrophage cell line (RAW264.7). This antioxidant property depends on concentration and increasing with increased amount of sample.