earticle

영어

Herein, we propose a novel methodology for efficient recovery of cell-free synthesized recombinant proteins. Through the expression of His-tagged proteins in the presence of Ni-NTA resin, the nascent polypeptides were instantly immobilized on the solid surface, thereby avoiding the inter-molecular aggregation. Upon the elution with imidazole, compared to the reactions
conducted without the resin, it was found that substantially higher amounts of soluble proteins were recovered most likely through improved folding the nascent polypeptides on the surface of resin. We expect that the proposed method will significantly contribute to rapid generation of properly folded proteins for numerous applications.