원문정보
초록
영어
In ion exchange chromatography for the recovery of potassium clavulanate from fermentation broth, a large amount of potassium chloride existed in the resulting solutions. In this study, diafiltration with nanofiltration membrane was employed to retain potassium clavulanate while removing chloride ions from the solutions. In the diafiltration of a solution containing 2.5 mM of potassium clavulanate and 1,000 mM of potassium chloride, almost complete removal of chloride was possible with no significant loss of clavulanate. However, clavulanate degradation was observed, although not very serious. Based on the experimental results of diafiltration, three different mathematical models were developed. The effect of temperature on clavulanate degradation was also modeled and was incorporated into those diafiltration models. Model predictions of clavulanate concentration and chloride concentration were in good agreement with the experimental data.