원문정보
초록
영어
Melissa officinalis is perennial herb occurring throughout the east Asia, which have been used for various traditional medicinal treatment. This plant is known to mainly possess rosmarinic acid and protocatechuic acid as polyphnolic acids. To investigate the biological activity of the flavonoids in these plants, it is necessary to develop an effective system for extracting and purifying the flavonoids with possible biological functions. Therefore, in this study, the extraction process with operating variable such as temperature, solvent type and concentration, and solid to solvent ratio was optimized by response surface analysis methodology. Also, the chromatographic purification of rosmarinic acid in the extract obtained from the solvent extraction was carried out by using adsorption chromatography resins such as XADs and Sephadex
LH-20 at a variety of conditions. In conclusion, this work is used as basic information for developing an effective purification strategy of rosmarinic acid from plant resources.