원문정보
초록
영어
Homoharringthonine was purified from Cephalotaxus koreana1-2) by a combination of extraction, synthetic adsorbent treatment, low-pressure chromatography, and high-performance liquid chromatography(HPLC). A crude extract was obtained by methanol extraction of biomass, followed by liquid-liquid extraction using chloroform. The waxy compounds were efficiently
removed by adsorbent(active clay P-1) treatment. The extract was purified to greater than 52% with 86.4% step yield by silica gel low-pressure chromatography. High-performance liquid chromatography steps, which were composed of an HPLC step with silica column and an HPLC step with ODS column, were applied to give 98% purity with high yield. Amorphous homoharringtonine, with a fine particle size, was simply made by dissolving homoharringtonine in methylene chloride/methanol(98/2, v/v), followed by spray drying. Residual solvents, methylene chloride and methanol were easily removed by to less than concentration limit in ICH guidance by spray drying. Amorphous homoharringtonine was markedly more soluble in water than crystalline homoharringtonine. This information is very useful for production and
quality control for pharmaceutical in commercialization step.