원문정보
초록
영어
Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from plant cell cultures. The pH of fractional precipitation was optimized in terms of the yield and purity of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v), and storage time (3 days). The maximum yield and purity of paclitaxel were obtained at pH 8.0 with potassium phosphate buffer. At a constant storage temperature (4℃), both the precipitate yield and purity increased rapidly up to 18 h, and then increased more slowly. This pre-purification process serves to minimize solvent usage, and the size and complexity of the high-performance liquid chromatography (HPLC) operation required for paclitaxel purification.