원문정보
초록
영어
β-Carotene synthesis plasmid pT-HB was used for cotransformation with pSdxs and pSSN12Didi which increased IPP and DMAPP synthesis. β-Carotene production significantly increased 1.5-fold with the amplification of the dxs gene through pSdxs and 4-fold with the mevalonate bottom pathway of pSSN12Didi in the presence of 3.3 mM mevalonate. The pT-DHB, constructed by integrating the dxs gene into pT-HB, was used for co-transformation of E. coli harboring
pSSN12Did, resulting into β-carotene production of 141 mg/L. Recombinant E. coli harboring pT-DHB and pSSN12Didi was used to maximize β-carotene production by adjusting the available amounts of glycerol, a carbon source, and mevalonate, the precursor of the mevalonate bottom pathway. When recombinant E. coli was given 16.5 mM mevalonate and 2.5% (w/v) glycerol, β-carotene production of 503 mg/L in concentration was obtained at 144 hours. This work was supported by the EB-NCRC (Grant No. R15-2003-012-02001-0), BioGreen 21 Program (Grant No. 2005041034590), Brain Pool program and BK21 program of Korea.