earticle

영어

Aptamers are ssDNA or RNA oligonucleotides bind to a wide range of target molecules with high affinity and specificity and they are applicated useful sensing molecule. The target, RBP4 secreted from adipocytes is known to be potential biomarker for type 2 diabetes. RBP4 aptamers have been selected by FluMag-SELEX process from random DNA library, composed of approximately 3.0 x 1016 DNA molecules. To analyze aptamers' affinity and specificity for
RBP4, Surface Plasmon Resonance(SPR) was used. No.38 aptamer was found to show highest specificity with low Kd value 226 nM among others. Detection of the target protein using aptamer was compared with Western blotting analysis and ELISA Assay for serum samples. The data shows that this aptamer biosensor is specific and effective for the detection of RBP4 in serum
samples and could be used as a diagnosis tool.