원문정보
초록
영어
A critical requirement for achieving a total micro-analytical system of cells and their constituent proteins is to develop the cell lysis and solid phase extraction (SPE) steps on-chip. This work was developed by miniaturized sample preparation systems, in which cell lysis was needed to obtain intracellular materials for analysis of intracellular protein. We adopt the micro-magnetic stirring method by an external magnetic stirrer for cell lysis of Escherichia coli. The intracellular sample and lysis buffer were well mixed by micro-magnetic stirring in a chamber, and the efficient-lysis with mixing time introduced into the SPE chamber, subsequently. We also analyzed the immobilization efficiency of intracellular protein after cell lysis onto the micro-patterned gold surface in microchamber. Using this microfluidic mixer system, we developed a
strategy for fabricating micro-patterned protein chip. The principle of this strategy is that the gold binding polypeptide (GBP) as a fusion partner is specifically immobilized onto the gold-patterned surface. The severe acute respiratory syndrome coronavirus envelope was used as a model protein for the specific immobilization of GBP-fusion proteins onto the gold-patterned surface. This micro-mixer fluidic system was combined with Lab-on-a-Chip applications on the gold surface and used for studying antigen-antibody interactions. [This work was supported in part by MIC & IITA through IT Leading R&D Support Project, by the KOSEF through the Center for
Ultramicrochemical Process Systems, and the Basic Research Program of the Korea Science and Engineering Foundation.]