원문정보
초록
영어
To detect bacterial pathogens, 16s rRNA was analyzed using a surface plasmon resonance (SPR) biosensor with a signal amplification technology. The 16s rRNA has been used as a genetic marker for identifying organism, and can be analyzed directly without PCR amplification due to relatively high copy number [1]. However, the direct detection of 16s rRNA shows sensitivity limitation compared with PCR-based assays. In this study, a signal enhancing method for high sensitive direct detection of bacterial 16S rRNA using peptide nucleic acid (PNA) probes was developed. The amount of hybridization was monitored by the SPR biosensor, which enables detection of molecular interactions on surface in response to changes in the index of refraction [2]. To amplify the interaction of PNA and 16s rRNA, surface-modified nanoparticles were designed to interact with 16s rRNA selectively. Using this amplification strategy, detection of various pathogens such as E. coli O157, Clostridium perfringens, Staphylococcus aureus was possible.