원문정보
초록
영어
A n-type field-effect-transistor (n-type FET) biosensor for detecting the protein-protein interaction on the sensing layer was fabricated. Silicon dioxide (SiO2) of the gate oxide was used as the layer to detect the protein. As a platform model, we have used C-reactive protein (CRP) which is a cardiac and inflammation marker. The reagents such as APTS, poly(ethylene glycol) iglycidyl ether were used to bind with amine portion of the anti-CRP. After binding the anti-CRP on the sensing layer, CRP was injected. The binding reaction of the anti-CRP and CRP was detected to the electrical signal by FET-type biosensor. The measurement was performed in phosphate buffer saline (PBS;pH 7.4) solution and Pt was used as the reference electrode. The response
of the anti-CRP and CRP have caused the variation of the drain current of a FET-type biosensor. To verify the interactions between the anti-CRP and CRP on the sensing layer, surface plasmon resonance (SPR) measurement has been performed. We confirmed that FET-type biosensor can detect the characterization of the protein-protein binding reaction using the real-time system.