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Background and Objectives The canal wall down mastoidectomy brings changes in theanatomy of the external auditory canal (EAC), causing potential problems, such as accumulat-ed crust, vertigo attacks, and difficulties in wearing hearing aids (HAs). The objective of thisstudy is to evaluate the safety and efficacy of mastoidoplasty using the demineralized bonematrix (DBM) to obliterate the mastoidectomized cavity and reconstruct EAC. Subjects and Method Medical records of patients with chronic otitis media with or withoutcholesteatoma who received mastoidoplasty using DBM by a single surgeon at Seoul St. Mary’shospital between 2014 and 2021 were reviewed retrospectively. Results A total of 27 patients were included in this study. None of the patients showed anyrecurrence of cavity problem, wound infection, or any other complications during their follow-up period of 13.07±37 months. The average air and bone conduction hearing level of puretone audiometry showed no significant change after surgery ( p=0.50, p=0.54, respectively). Five patients indicated for hearing rehabilitation could adopt canal type HAs after surgery; sixpatients used completely-in-the canal type HAs, and one patient used in-the-canal type HAs. None of the patients using HAs complained of acoustic feedback or any other problem inwearing HAs. Conclusion Mastoidoplasty using DBM seems to be a very safe and effective surgical pro-cedure that shows functionally acceptable EAC for hearing rehabilitation with canal type HAsand demonstrates no specific complication.