@article{ART002916984,
author={Wu Szu-Hsien Sam and Lee Heetak and Szép-Bakonyi Réka and Colozza Gabriele and Boese Ayse and Gert Krista R. and Hallay Natalia and Lee Ji-Hyun and Kim Jihoon and Zhu Yi and Linssen Margot M. and Pilat-Carotta Sandra and Hohenstein Peter and Theussl Hans-Christian and Pauli Andrea and Koo Bon-Kyoung},
title={SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection},
journal={Experimental and Molecular Medicine},
issn={1226-3613},
year={2022},
volume={54},
pages={2188-2199},
doi={10.1038/s12276-022-00891-0}
}

TY - JOUR
AU - Wu Szu-Hsien Sam
AU - Lee Heetak
AU - Szép-Bakonyi Réka
AU - Colozza Gabriele
AU - Boese Ayse
AU - Gert Krista R.
AU - Hallay Natalia
AU - Lee Ji-Hyun
AU - Kim Jihoon
AU - Zhu Yi
AU - Linssen Margot M.
AU - Pilat-Carotta Sandra
AU - Hohenstein Peter
AU - Theussl Hans-Christian
AU - Pauli Andrea
AU - Koo Bon-Kyoung
TI - SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
T2 - Experimental and Molecular Medicine
JO - Experimental and Molecular Medicine
PY - 2022
VL - 54
IS - null
PB - 생화학분자생물학회
SP - 2188
EP - 2199
SN - 1226-3613
AB - The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.
KW -
DO - 10.1038/s12276-022-00891-0
ER -

Wu Szu-Hsien Sam, Lee Heetak, Szép-Bakonyi Réka, Colozza Gabriele, Boese Ayse, Gert Krista R., Hallay Natalia, Lee Ji-Hyun, Kim Jihoon, Zhu Yi, Linssen Margot M., Pilat-Carotta Sandra, Hohenstein Peter, Theussl Hans-Christian, Pauli Andrea and Koo Bon-Kyoung. 2022, "SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection", Experimental and Molecular Medicine, vol.54, pp.2188-2199. Available from: doi:10.1038/s12276-022-00891-0

Wu Szu-Hsien Sam, Lee Heetak, Szép-Bakonyi Réka, Colozza Gabriele, Boese Ayse, Gert Krista R., Hallay Natalia, Lee Ji-Hyun, Kim Jihoon, Zhu Yi, Linssen Margot M., Pilat-Carotta Sandra, Hohenstein Peter, Theussl Hans-Christian, Pauli Andrea, Koo Bon-Kyoung "SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection" Experimental and Molecular Medicine 54 pp.2188-2199 (2022) : 2188.

Wu Szu-Hsien Sam, Lee Heetak, Szép-Bakonyi Réka, Colozza Gabriele, Boese Ayse, Gert Krista R., Hallay Natalia, Lee Ji-Hyun, Kim Jihoon, Zhu Yi, Linssen Margot M., Pilat-Carotta Sandra, Hohenstein Peter, Theussl Hans-Christian, Pauli Andrea, Koo Bon-Kyoung. SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection. 2022; 54 2188-2199. Available from: doi:10.1038/s12276-022-00891-0

Wu Szu-Hsien Sam, Lee Heetak, Szép-Bakonyi Réka, Colozza Gabriele, Boese Ayse, Gert Krista R., Hallay Natalia, Lee Ji-Hyun, Kim Jihoon, Zhu Yi, Linssen Margot M., Pilat-Carotta Sandra, Hohenstein Peter, Theussl Hans-Christian, Pauli Andrea and Koo Bon-Kyoung. "SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection" Experimental and Molecular Medicine 54(2022) : 2188-2199.doi: 10.1038/s12276-022-00891-0

Wu Szu-Hsien Sam; Lee Heetak; Szép-Bakonyi Réka; Colozza Gabriele; Boese Ayse; Gert Krista R.; Hallay Natalia; Lee Ji-Hyun; Kim Jihoon; Zhu Yi; Linssen Margot M.; Pilat-Carotta Sandra; Hohenstein Peter; Theussl Hans-Christian; Pauli Andrea; Koo Bon-Kyoung. SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection. Experimental and Molecular Medicine, 54, 2188-2199. doi: 10.1038/s12276-022-00891-0

Wu Szu-Hsien Sam; Lee Heetak; Szép-Bakonyi Réka; Colozza Gabriele; Boese Ayse; Gert Krista R.; Hallay Natalia; Lee Ji-Hyun; Kim Jihoon; Zhu Yi; Linssen Margot M.; Pilat-Carotta Sandra; Hohenstein Peter; Theussl Hans-Christian; Pauli Andrea; Koo Bon-Kyoung. SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection. Experimental and Molecular Medicine. 2022; 54 2188-2199. doi: 10.1038/s12276-022-00891-0

Wu Szu-Hsien Sam, Lee Heetak, Szép-Bakonyi Réka, Colozza Gabriele, Boese Ayse, Gert Krista R., Hallay Natalia, Lee Ji-Hyun, Kim Jihoon, Zhu Yi, Linssen Margot M., Pilat-Carotta Sandra, Hohenstein Peter, Theussl Hans-Christian, Pauli Andrea, Koo Bon-Kyoung. SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection. 2022; 54 2188-2199. Available from: doi:10.1038/s12276-022-00891-0

Wu Szu-Hsien Sam, Lee Heetak, Szép-Bakonyi Réka, Colozza Gabriele, Boese Ayse, Gert Krista R., Hallay Natalia, Lee Ji-Hyun, Kim Jihoon, Zhu Yi, Linssen Margot M., Pilat-Carotta Sandra, Hohenstein Peter, Theussl Hans-Christian, Pauli Andrea and Koo Bon-Kyoung. "SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection" Experimental and Molecular Medicine 54(2022) : 2188-2199.doi: 10.1038/s12276-022-00891-0