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This study aimed to investigate the antioxidant activity of Tradescantia palida methanol extract (TPME) and its effect on viability of UVA-irradiated human dermal fibroblasts (HDFs). The total polyphenol and flavonoid contents of TPME were 418.4 tannic acid equivalent (TAE) mg/g, and 247.1 rutin equivalent (RE) mg/g, respectively. The electron-donating abilities of TPME and ascorbic acid (AA) at 1,000 μg/mL were 66.8% and 97.5%, respectively. The 50% scavenging concentration of free radicals (SC50) from TPME and AA were 660.8 μg/mL and <125 μg/mL, respectively. The maximum permissible concentration of TPME in treating HDFs was 50 μg/mL. Treatment of 10, 15, and 20 mJ/cm2 UVA-irradiated HDFs with 50 μg/mL TPME increased cellular viability by 5.40% (p<0.01), 15.0% (p<0.001), and 20.8% (p<0.001), respectively. Treatment with 50 μg/mL AA increased cellular viability by 11.5% (p<0.001), 20.6% (p<0.001), and 15.4% (p<0.001) in 10, 15, and 20 mJ/cm2 UVA-irradiated HDFs, respectively. These results indicate that TPME contains abundant antioxidants, which confer TPME with a high degree of antioxidant activity. Thus, TPME is a natural antioxidant that can protect HDFs against UVA-irradiation.
