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Background/Aims: Diagnostic abdominal paracentesis has been described in literature to have variable sensitivity of 50%–75% forthe detection of peritoneal carcinomatosis (PC). We believe that random needle aspirates from the omentum, even in the absence ofobvious deposits by endoscopic ultrasound (EUS), could prove malignancy in patients with PC. Methods: Consecutive patients who underwent EUS for diagnosis and staging of cancer and found to have ascites were includedafter obtaining informed consent. EUS-guided fine needle aspiration (EUS-FNA) from random sites in the omentum was performedthrough the transgastric route using a linear echoendoscope. Results: Fifty-four patients underwent EUS during October 2015 to April 2017 for detection, staging, or FNA of a suspectedmalignant lesion. Ascites was seen in 17 patients and 15 patients who fulfilled the criteria were included. The procedure wassuccessful in all patients. Cytology was suggestive of malignancy in 12 (80%) but not suggestive of malignancy in 3 (20%) patients. Three patients who tested negative had hyperbilirubinemia with biliary obstruction. Their ascitic fluid analysis result was alsonegative. Conclusions: Random FNA of the omentum in patients with malignancy-related ascites is highly effective in the diagnosis of PC andcould be employed during EUS evaluation of malignancies.