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The aim of this study is to investigate the antioxidant activity of Hericium erinaceum grown on hulled barley (HEHB), and to develop a potential substitute tea using the extract of HEHB. The antioxidant activities of HEHB and HB extracts were assessed using various 1.1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid (ABTS+) radical scavenging assays, as well as nitrite scavenging activity, reducing power and linoleic acid oxidation inhibition assays. The total phenolic and flavonoid contents of the HEHB water extract were increased compared to those of the hulled barley (HB) water extract. Moreover, the HEHB water extracts showed a higher scavenging activity on DPPH and ABTS+ radicals and nitrite. Also, the reducing power increased with increasing concentration of the HEHB water extract. In addition, HEHB water extracts showed higher linoleic acid oxidation inhibition activity than the HB water extract. In conclusion, HBHE might have the potential to be used as a substitute tea materials with good antioxidant activity.