초록 열기/닫기 버튼
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Apoptosis is a morphologically distinct form of programed cell death that plays that a key role in the growth regulation of a variety of tissues. Several techniques based on the detection of DNA fragmentation as an important characteristic of this process have been established. Since their methods, however, are expensive, time-consuming, and poor tissue morphology, it can be difficulty to apply in routine histological sections. The aim of this study was performed to determine the usefulness of toluidine blue staining in Carnoy-fixed and paraffin-embedded tissues. The samples from spleen, small intestine, liver, kidney and testis was evaluated. We compared the toluidine blue (TB) staining with the hematoxylin and eosin (HE) and terminal deoxylnucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) staining used to detection of Apoptotic cells. The distribution of Apoptotic cells in five tissues was similar to the site of previous studies. The Apoptotic cells are classified as early and late phase based on their morphology. In HE sections, apoptosis was usually recognizable by the presence of apoptotic bodies (late phase). Although the early phase of apoptosis can be detected as a cells possessing an nucleus with irregular contour and condensed chromatin magination at nuclear periphery in HE sections, it was difficult to detect and was easily overlooked. In TUNEL sections, apoptosis appeared intense staining mainly in late phase and weak staining in early phase. However, the colorized signals of TUNEL are much easier to detect than apoptotic bodies in HE sections. In TB staining cells revealed with metachromatic staining in early phase and late phase with hyperchromatic staining, though the intensity of metachromatic staining is less weakened than positive signals in TUNEL, it can be discriminated between early and late apoptotic cells. Thus, if the metachromatic intensity of TB, in future, could be more improved, it may be a cheap, convenient, useful technique for detection of apoptosis as well as for discrimination of early and late apoptotic cells in Carnoy-fixed and paraffin-embedded tissues.
키워드열기/닫기 버튼
Apoptosis, Toluiding blue staining, Paraffin-embedded tissues
