초록 열기/닫기 버튼
In this study, we investigated the efects of PAHs and dioxin on mRNA and plasma protein expresion using genomic and proteomic ana-lysis for automobile emision inspectors and waste incineration workers. About 54 workers from automobile emission inspection ofices, 31 workers from waste incinerating company and 84 unexposed healthy subjects were enroled in this study. Urine and air samples were colected says were caried out to evaluate any DNA damage in mononuclear and polynuclear cels. A significant diference in Olive tail moments in mononuclear cels was observed between ex-posed and control subjects (P＜ 0.0001). To examine the diferences of the gene expresion profile in automobile emission inspectors and waste incineration workers, radioactive comple-mentary DNA microarays were used to evaluate The gene expression profiles showed that 11 genes were up-regulated and 4 genes were down-regulated in waste incinerating workers as compared with controls. Plasma proteins were analyzed by 2-dimentional electrophoresis with pH 3-10 NL IPG Dry strip. The protein expres-sion profiles showed that 8 proteins were up- regulated and 1 protein, haptoglobin, was down- regulated in automobile emision inspectors and waste incineration workers. Serum paraoxonase/ arylesterase was found only in the plasma of genes and proteins involved in oxidative stres were up-regulated in both automobile emission inspectors and waste incineration workers. Se-veral proteins, such as transthyrethin, sarcol-ectin and haptoglobin, that were highly up- or down-regulated, could serve as biological moni-toring markers for future study.
키워드열기/닫기 버튼
biological monitoring markers; dioxins; genomic; polycyclic aromatic hydrocarbons; proteomic
