@article{ART001055788,
author={강봉선 and 안진영 and 김민기 and 김현정 and 강라미 and 임헌창 and 박경숙 and 이재선 and 서정선 and 차중익 and 김승업 and 박유정 and 김만호},
title={Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin},
journal={Experimental and Molecular Medicine},
issn={1226-3613},
year={2007},
volume={39},
number={1},
pages={38-46}
}

TY - JOUR
AU - 강봉선
AU - 안진영
AU - 김민기
AU - 김현정
AU - 강라미
AU - 임헌창
AU - 박경숙
AU - 이재선
AU - 서정선
AU - 차중익
AU - 김승업
AU - 박유정
AU - 김만호
TI - Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin
T2 - Experimental and Molecular Medicine
JO - Experimental and Molecular Medicine
PY - 2007
VL - 39
IS - 1
PB - 생화학분자생물학회
SP - 38
EP - 46
SN - 1226-3613
AB - Huntingtons disease is caused by CAG trinucleotide expansions in the gene encoding huntingtin. N- terminal fragments of huntingtin with polyglu-tamine produce aggregates in the endosome-ly-sosomal system, where proteolytic fragments of huntingtin is generated. Heat shock protein 70 (HSP70) prevents the formation of protein aggre-gates, but the effect of HSP70 on the huntingtin in the endosome-lysosomal system is unknown. This study was to determine whether HSP70 alters the distribution of huntingtin in endosome-lysosomal system. HSP70 expressing stable cells (NIH/3T3 or cerebral hybrid cell line A1) were generated, and mutant [(CAG)100] huntingtin was transiently over-expressed. Analysis of subcellular distribution by immnuocytochemistry or proteolysis cleavage by Western blotting was performed. 18 CAG repeat wild type [WT; (CAG)18] huntingtin was used as a control. Cells with huntingtin showed paterns of endosome- lysosomal accumulation, from a dispersed vacuole (DV) type into a coalescent perinuclear vacuole (PV) type over time. In WT huntingtin, HSP70 increased the cells with the PV types that enhanced the proteolytic cleavage of huntingtin. However, HSP70 reduced cells of the DV and PV types expressing mutant ess proteolysis than that of control. In addition, intranuclear inclusions were formed only in mutant cells, which was no t affected by HSP70. These results suggest that HSP70 alters the distribution of huntingtin in the endosome- lysosomal system, and that this contributes to huntingtin proteolysis.
KW - endosomes; HSP70 heat-shock proteins; Huntington disease; lysosome
DO -
UR -
ER -

강봉선, 안진영, 김민기, 김현정, 강라미, 임헌창, 박경숙, 이재선, 서정선, 차중익, 김승업, 박유정 and 김만호. 2007, "Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin", Experimental and Molecular Medicine, vol.39, no.1 pp.38-46.

강봉선, 안진영, 김민기, 김현정, 강라미, 임헌창, 박경숙, 이재선, 서정선, 차중익, 김승업, 박유정, 김만호 "Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin" Experimental and Molecular Medicine 39.1 pp.38-46 (2007) : 38.

강봉선, 안진영, 김민기, 김현정, 강라미, 임헌창, 박경숙, 이재선, 서정선, 차중익, 김승업, 박유정, 김만호. Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin. 2007; 39(1), 38-46.

강봉선, 안진영, 김민기, 김현정, 강라미, 임헌창, 박경숙, 이재선, 서정선, 차중익, 김승업, 박유정 and 김만호. "Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin" Experimental and Molecular Medicine 39, no.1 (2007) : 38-46.

강봉선; 안진영; 김민기; 김현정; 강라미; 임헌창; 박경숙; 이재선; 서정선; 차중익; 김승업; 박유정; 김만호. Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin. Experimental and Molecular Medicine, 39(1), 38-46.

강봉선; 안진영; 김민기; 김현정; 강라미; 임헌창; 박경숙; 이재선; 서정선; 차중익; 김승업; 박유정; 김만호. Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin. Experimental and Molecular Medicine. 2007; 39(1) 38-46.

강봉선, 안진영, 김민기, 김현정, 강라미, 임헌창, 박경숙, 이재선, 서정선, 차중익, 김승업, 박유정, 김만호. Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin. 2007; 39(1), 38-46.

강봉선, 안진영, 김민기, 김현정, 강라미, 임헌창, 박경숙, 이재선, 서정선, 차중익, 김승업, 박유정 and 김만호. "Heat shock protein 70 alters the endosome-lysosomallocalization of huntingtin" Experimental and Molecular Medicine 39, no.1 (2007) : 38-46.