@article{ART001897261,
author={Jie Jia and Xiaobo Feng and Weihua Xu and Shu-Hua Yang and Qing Zhang and Xianzhe Liu and Yong Feng and Zhipeng Dai},
title={MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis},
journal={Experimental and Molecular Medicine},
issn={1226-3613},
year={2014},
volume={46},
pages={1-8}
}

TY - JOUR
AU - Jie Jia
AU - Xiaobo Feng
AU - Weihua Xu
AU - Shu-Hua Yang
AU - Qing Zhang
AU - Xianzhe Liu
AU - Yong Feng
AU - Zhipeng Dai
TI - MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis
T2 - Experimental and Molecular Medicine
JO - Experimental and Molecular Medicine
PY - 2014
VL - 46
IS - null
PB - 생화학분자생물학회
SP - 1
EP - 8
SN - 1226-3613
AB - MicroRNAs (miRNAs) have recently been recognized to have a role in human orthopedic disorders. The objective of our study was to explore the expression profile and biological function of miRNA-17-5p (miR-17-5p), which is well known to be related to cancer cell proliferation and invasion, in osteoblastic differentiation and in cell proliferation. The expression levels of miR-17- 5p in the femoral head mesenchymal stem cells of 20 patients with non-traumatic osteonecrosis (ON) and 10 patients with osteoarthritis (OA) were examined by quantitative reverse transcription-PCR (qRT–PCR). Furthermore, the interaction between miR-17-5p and SMAD7 was observed. We found that in non-traumatic ON samples the level of mature miR-17-5p wassignificantly lower than that of OA samples (P¼0.0002). By targeting SMAD7, miR-17-5p promoted nuclear translocation of b-catenin, enhanced expression of COL1A1 and finally facilitated the proliferation and differentiation of HMSC-bm cells. We also demonstrated that restoring expression of SMAD7 in HMSC-bm cells partially reversed the function of miR-17-5p. Together, our data suggested a theory that dysfunction of a network containing miR-17-5p, SMAD7 and b-catenin could contribute to ON pathogenesis. The present study prompts the potential clinical value of miR-17-5p in non-traumatic ON.
KW -
DO -
UR -
ER -

Jie Jia, Xiaobo Feng, Weihua Xu, Shu-Hua Yang, Qing Zhang, Xianzhe Liu, Yong Feng and Zhipeng Dai. 2014, "MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis", Experimental and Molecular Medicine, vol.46, pp.1-8.

Jie Jia, Xiaobo Feng, Weihua Xu, Shu-Hua Yang, Qing Zhang, Xianzhe Liu, Yong Feng, Zhipeng Dai "MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis" Experimental and Molecular Medicine 46 pp.1-8 (2014) : 1.

Jie Jia, Xiaobo Feng, Weihua Xu, Shu-Hua Yang, Qing Zhang, Xianzhe Liu, Yong Feng, Zhipeng Dai. MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis. 2014; 46 1-8.

Jie Jia, Xiaobo Feng, Weihua Xu, Shu-Hua Yang, Qing Zhang, Xianzhe Liu, Yong Feng and Zhipeng Dai. "MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis" Experimental and Molecular Medicine 46(2014) : 1-8.

Jie Jia; Xiaobo Feng; Weihua Xu; Shu-Hua Yang; Qing Zhang; Xianzhe Liu; Yong Feng; Zhipeng Dai. MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis. Experimental and Molecular Medicine, 46, 1-8.

Jie Jia; Xiaobo Feng; Weihua Xu; Shu-Hua Yang; Qing Zhang; Xianzhe Liu; Yong Feng; Zhipeng Dai. MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis. Experimental and Molecular Medicine. 2014; 46 1-8.

Jie Jia, Xiaobo Feng, Weihua Xu, Shu-Hua Yang, Qing Zhang, Xianzhe Liu, Yong Feng, Zhipeng Dai. MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis. 2014; 46 1-8.

Jie Jia, Xiaobo Feng, Weihua Xu, Shu-Hua Yang, Qing Zhang, Xianzhe Liu, Yong Feng and Zhipeng Dai. "MiR-17-5p modulates osteoblastic differentiation and cell proliferation by targeting SMAD7 in non-traumatic osteonecrosis" Experimental and Molecular Medicine 46(2014) : 1-8.